Description
Aviva Systems Biology Serpina11 ELISA Kit (Mouse) (OKEH06344) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Serpina11 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Serpina11 and incubated. The Serpina11 found in the sample or standards competes with the biotinylated Serpina11 for limited binding sites on the immobilized anti-Serpina11 antibody. Excess unbound biotinylated Serpina11 and sample or standard Serpina11 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Serpina11 captured in the well and inversely proportional to the amount of Serpina11 which was contained in the sample or standard